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Journal

Reverse Mode of the Sodium/Calcium Exchanger Subtype 3 in Interstitial Cells of Cajal From Rat Bladder

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Published:25th Mar 2020
Source: Urology
Objective: To investigate how the sodium/calcium exchanger subtype 3 (NCX3) and its reverse mode contribute to the function of interstitial cells of Cajal (ICCs) from the rat bladder. Methods: The study used 20 female Wistar rats. We observed the expression of the NCX3 expression in the bladder using reverse transcriptase-polymerase chain reaction and Western blotting. The NCX3 in ICCs was also confirmed by double-labeled fluorescence. NCX3 functions in reverse mode of ICCs were observed using confocal microscopy with preload fluo-3AM, and its currents were evaluated using the whole-cell patch clamp technique, with or without the NCX3 inhibitor KB-R7943 (5 and 30?M), with an afterward identification of ICCs using single-cell polymerase chain reaction. Results: NCX3 was confirmed in rat bladder ICCs. The time required for the intracellular calcium concentration [Ca2+]i of NCX3 was enhanced by KB-R7943 (5?M, P ?.01). Moreover, KB-R7943 (5 and 30?M) significantly decreased the currents generated by the reverse mode of NCX3 from the ICCs (P <.05). conclusion: ncx3 is expressed in rat bladder iccs. the reverse mode of ncx3 can generate [ca2+]i of the bladder iccs.>

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