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Guideline

Guidelines on the laboratory aspects of assays used in haemostasis and thrombosis

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Last updated:1st Feb 2013

Assays of procoagulant and anticoagulant factors in plasma are required to diagnose haemostatic abnormalities and to monitor treatment of replacement therapy. Well designed and controlled assays are important to ensure accurate and precise results are obtained.

This guideline covers details of general assay design, including pre-analytical variables, reference ranges and cut-off values, and assay techniques and reagents. More specific detail is given for measurement of procoagulant clotting factors, and thrombophilia screening through assay of the natural anticoagulants antithrombin, protein C and protein S.

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